LOCUS       40924_19021        3465 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pF12K RM Flexi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Vidugiriene J, Schenborn E, McNamara B.
  TITLE     Regulated Mammalian Expression System Technical Manual, TM289
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Vidugiriene J, Schenborn E, McNamara B.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2006) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 3465)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3465)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-SEP-2006) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Road, Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..309
                     /label=12X lambda operator
                     /note="12X lambda operator"
     regulatory      322..434
                     /label=minimal CMV promoter
                     /note="minimal CMV promoter"
                     /regulatory_class="promoter"
     CDS             477..809
                     /label=barnase
                     /note="ribonuclease from Bacillus amyloliquefaciens"
     misc_feature    822..877
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     polyA_signal    complement(982..1103)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             1502..2293
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     rep_origin      complement(2468..3056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3172..3455
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
ORIGIN
        1 agtactccgc tcgagtttac ctctggcggt gatagtcgag tttacctctg gcggtgatag
       61 tcgagtttac ctctggcggt gatagtcgag tttacctctg gcggtgatag tcgagtttac
      121 ctctggcggt gatagtcgag tttacctctg gcggtgatag tcgagtttac ctctggcggt
      181 gatagtcgag tttacctctg gcggtgatag tcgagtttac ctctggcggt gatagtcgag
      241 tttacctctg gcggtgatag tcgagtttac ctctggcggt gatagtcgag tttacctctg
      301 gcggtgatag tcgactctag ataggcgtgt acggtgggag gcctatataa gcagagctgg
      361 tttagtgaac cgtcagatcc ctggagacgc catccacgct gttttgacct ccatagaaga
      421 caccgggacc gatcaaccta agcttgcgat cgccatggaa taagtaagga atccacatgg
      481 cacaggttat caacacgttt gacggggttg cggattatct tcagacatat cataagctac
      541 ctgataatta cattacaaaa tcagaagcac aagccctcgg ctgggtggca tcaaaaggga
      601 accttgcaga cgtcgctccg gggaaaagca tcggcggaga catcttctca aacagggaag
      661 gcaaactccc gggcaaaagc ggacgaacat ggcgtgaagc ggatattaac tatacatcag
      721 gcttcagaaa ttcagaccgg attctttact caagcgactg gctgatttac aaaacaacgg
      781 accattatca gacctttaca aaaatcagat aatgtttaaa cgaattcggg ctcggtaccc
      841 ggggatcctc tagagtcgac ctgcaggcat gcaagctgat ccggctgcta acaaagcccg
      901 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
      961 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1021 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1081 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1141 agggggagat gtgggaggtt tttttaagca agtaaaacct ctacaaatgt ggtaaaatcg
     1201 aattctaatg gatcctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga
     1261 cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtgttc cgcttccttt
     1321 agcagccctt gcgccctgag tgcttgcggc agcgtgagct tcaaaagaat tgccagctgg
     1381 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     1441 aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgac ggtcgtttcg
     1501 catgcttgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     1561 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     1621 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     1681 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     1741 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     1801 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     1861 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     1921 catcgagcga gcacgcactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     1981 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gtatgccgga
     2041 tggtgaggat ctcgtcgtga ctcatggcga tgcctgcttg ccgaatatca tggtggaaaa
     2101 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     2161 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     2221 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     2281 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     2341 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2401 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2461 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2521 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2581 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2641 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2701 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2761 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2821 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2881 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     2941 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3001 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tttcaagaag
     3061 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3121 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttatag tccggaaata
     3181 caggaacgca cgctggatgg cccttcgctg ggatggtgaa accatgaaaa atggcagctt
     3241 cagtggatta agtgggggta atgtggcctg taccctctgg ttgcataggt attcatacgg
     3301 ttaaaattta tcaggcgcga ttgcggcagt ttttcgggtg gtttgttgcc atttttacct
     3361 gtctgctgcc gtgatcgcgc tgaacgcgtt ttagcggtgc gtacaattaa gggattatgg
     3421 taaatccact tactgtctgc cctcgtagcc atcgagataa accgc
//
